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Agarose (Molecular biology grade) |
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Ordering Information: |
Cat. No. |
Pack |
Price (Rs.) |
3095 |
20gm |
3500 |
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Applications
• Routine DNA/RNA electrophoresis
• Immunology diffusion experiments
Gel point: 36ºC
Melting temperature: 88ºC
Storage
Store at room temperature
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Description
Agarose is suitable for DNA/RNA electrophoresis and also for immunology experiments. |
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Agarose-LM (Low melting agarose) |
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Ordering Information: |
Cat. No. |
Pack |
Price (Rs.) |
3177 |
10gm |
5200 |
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Applications
• Gel extractions of DNA fragments
• Experiments needs low melting temperature
Gel point: 24-26ºC
Melting temperature: 68ºC
Storage
Store at room temperature |
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Description
Low Melt Agarose (Agarose-LM) was designed for isolating DNA fragments from agarose. Due to its high gel strength, the agarose handles very well. The DNA band can be excised from the gel with a razor or scalpel, the gel fragment melted at 68°C, and then purified. This DNA may then be used for a variety of applications including restriction enzyme digestion, ligation and PCR. Alternatively, ligation may be performed directly in the melted agarose.
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6X Gel loading Dye |
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Ordering Information: |
Cat. No. |
Pack |
Price (Rs.) |
3097 |
5 X 1ml. |
600 |
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Applications
• Visual two color tracking of DNA migration during electrophoresis
• No DNA masking during gel exposure to UV light.
Usage Recommendation
Use 5 µl of 6X Gel Loading Dye per 25 µl reaction, or 10 µl per 50 µl reaction. Mix well before loading gel.
Storage
Store at 25ºC |
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Description
6X Gel Loading Dye is a pre-mixed loading buffer with two tracking dye for agarose and non-denaturing polyacrylamide gel electrophoresis. EDTA is also included to chelate magnesium (up to 10mM) in enzymatic reactions, thereby sTopping the reaction. Bromophenol blue and Xylene cynaol are the standard tracking dyes for electrophoresis. It migrates at approximately 300bp and 4000bp respectively on a standard 1% TBE agarose gel. |
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SuiteAll ® 6X Gel loading Dye |
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Ordering Information: |
Cat. No. |
Pack |
Price (Rs.) |
3178 |
5 X 1ml. |
1500 |
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Applications
• Any size DNA products can be tracked.
• Orange co-migrates with 50bp DNA fragments.
• Visual three color tracking of DNA migration
uring electrophoresis
• No DNA masking during gel exposure to UV light.
Usage Recommendation
Use 5 µl of 6X Gel Loading Dye per 25 µl reaction, or 10 µl per 50 µl reaction. Mix well before loading gel.
Storage
Store at 25ºC |
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Description
SuiteAll® 6X Gel Loading Dye is a pre-mixed loading buffer with three tracking dye for agarose and non-denaturing polyacrylamide gel electrophoresis and it contains three dyes, Bromophenol blue, xylene cyanol and Orange G. The orange migrates faster than Bromophenol blue and xylene cyanol. |
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6X Gel loading Dye with SDS |
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Ordering Information: |
Cat. No. |
Pack |
Price (Rs.) |
3179 |
5 X 1ml. |
1800 |
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Applications
• Sharp bands when used with Restriction digested DNA samples.
• Visual three color tracking of DNA migration during electrophoresis.
• No DNA masking during gel exposure to UV light.
Usage Recommendation
Use 5 µl of 6X Gel Loading Dye per 25 µl reaction, or 10 µl per 50 µl reaction. Mix well before loading gel.
Storage
Store at 25ºC
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Description
6X Gel Loading Dye is a pre-mixed loading buffer with two tracking dye for agarose and non-denaturing polyacrylamide gel electrophoresis. This solution contains SDS, which often results in sharper bands, as some restriction enzymes are known to remain bound to DNA following cleavage. EDTA is also included to chelate magnesium (up to 10 mM) in enzymatic reactions, thereby sTopping the reaction. |
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10X SafeBlue DNA stain |
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Ordering Information: |
Cat. No. |
Pack |
Price (Rs.) |
3098 |
100ml |
1100 |
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Sensitivity
100ng
Usage Recommendation
• Prepare 50ml of 1X SafeBlue DNA stain and incubate gel in stain for 1 hour.
• Wash gel thrice with distilled water.
• Observe gel in white light. A DNA band appears as a dark blue color against light blue gel background.
Storage
Store at room temperature
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Description
10X SafeBlue DNA stain is user friendly stain to stain DNA in gel. DNA bands can be visually seen without UV transilluminator.
Applications
• Suitable for schools & Colleges
• User friendly stain
• DNA bands can be seen without UV transilluminator.
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GreenSafe® DNA stain (Ready to use) |
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Ordering Information: |
Cat. No. |
Pack |
Price (Rs.) |
3180 |
0.5ml |
750 |
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Sensitivity
50ng
Usage Recommendation
Use 5µl GreenSafe® DNA stain for 100ml of agarose gel.
Storage
Store at room temperature
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Description
GreenSafe® DNA stain is user friendly stain to stain DNA in gel. DNA bands appear green color when seeing in UV-Transilluminator.
Applications
• Best alternate to Ethidium bromide
• Non-carcinogenic
• User friendly stain
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Ethidium bromide (Ready to use) |
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Ordering Information: |
Cat. No. |
Pack |
Price (Rs.) |
3099 |
1ml |
750 |
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Usage Recommendation
Use 5µl per 100ml of Agarose gel, mix well and pour into gel casting tray.
Caution:
Carcinogenic/Handle care
Use gloves for handling
Storage
Store at 4ºC or room temperature
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Description
Ethidium Bromide is a highly pure, DNase/RNase free product graded for Biotech applications. It is as solutions for added safety and convenience, at 10mg/ml.
Applications
• used for nucleic acid staining after gel electrophoresis
• Ready to use concentration.
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10X TE |
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Ordering Information: |
Cat. No. |
Pack |
Price (Rs.) |
3167 |
100ml |
1200 |
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Usage Recommendation
Dilute 10 X TE buffer 9:1 with distilled water for ready to use solution.
Storage
Store at room temperature
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Description
TE buffer is commonly used buffer solution in molecular biology, especially in procedures involving DNA or RNA. TE buffer is provided as a 50x concentrate, after diluting 49:1 in reagent grade water the working solution contains 10mM Tris and 1mM EDTA. The purpose of TE buffer is to protect DNA or RNA from degradation
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50X TAE |
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Ordering Information: |
Cat. No. |
Pack |
Price (Rs.) |
3154 |
100ml |
800 |
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Composition
The working solution contains 40mM Tris, 1.14ml/L Acetic Acid, and 2mM EDTA.
Usage Recommendation
Dilute 50 X TAE buffer 49:1 with distilled water for working solution.
Storage
Store at room temperature |
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Description
TAE buffer is a buffer solution used in agarose electrophoresis, typically for the separation of nucleic acids such as DNA and RNA. It is made up of Tris-acetate buffer, usually at pH 8.0, and EDTA, which sequesters divalent cations.
Specially formulated and filtered to ensure that they are of the highest possible quality, longest stability, and guaranteed to be free of contaminants.
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50X TBE |
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Ordering Information: |
Cat. No. |
Pack |
Price (Rs.) |
3096 |
50ml |
750 |
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Composition
The working solution contains 89mM Tris, 89mM Boric Acid and 2mM EDTA
Usage Recommendation
Dilute 50 X TBE buffer 49:1 with distilled water for working solution.
Storage
Store at room temperature
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Description
TBE buffer is a buffer solution used in agarose electrophoresis, typically for the separation of nucleic acids such as DNA and RNA. It is made up of Tris-borate buffer, usually at pH 8.0, and EDTA, which sequesters divalent cations.
Specially formulated and filtered to ensure that they are of the highest possible quality, longest stability, and guaranteed to be free of contaminants.
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20X FastRun® Buffer |
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Ordering Information: |
Cat. No. |
Pack |
Price (Rs.) |
3182 |
100ml |
1200 |
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Usage Recommendation
Dilute 20 X FastRun® buffer 19:1 with distilled water for working solution.
Storage
Store at room temperature
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Description
20X FastRun® is a suitable buffer for fast separation of DNA Fragments in agarose electrophoresis. Resolutions are better even run in high voltage because of its high buffering capacity. It is made up of sodium borate usually at pH 8.0.
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10X MOPS Buffer |
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Ordering Information: |
Cat. No. |
Pack |
Price (Rs.) |
3183 |
100ml |
2000 |
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Usage Recommendation
Dilute 10 X MOPS buffer 9:1 with distilled water for working solution.
Storage
10X MOPS-EDTA-Na Acetate Buffer can be stored for 3 months at room temperature. Avoid exposure to light and moisture. |
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Description
MOPS (3-Morpholinopropanesulfonic acid)-EDTA-Sodium Acetate Buffer is especially designed for agarose gel electrophoresis of RNA. The buffer is supplied at a 10x concentration and is ready for use. For optimal denaturation and visualisation of samples we recommend HELINI RNA Loading Buffers. |
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2X RNA Gel loading Buffer |
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Ordering Information: |
Cat. No. |
Pack |
Price (Rs.) |
3184 |
5 X 1ml |
1950 |
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Usage Recommendation
Add an equal volume of 2X RNA loading buffer to RNA sample and mix well
Heat the mixture at 70ºC for 10min
Chill on ice and spin down prior to loading on a gel.
Storage
Store at 4ºC |
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Description
2X RNA Gel loading buffer is especially designed for agarose gel electrophoresis of RNA. The buffer is supplied at a 2X concentration and is ready for use. It contains Bromophenol blue, xylene cyanol and the intercalating agent ethidium bromide. Formamide is added to stabilize RNA. |
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