Description:
Purified normal human IgG is isolated using fractionation and DEAE chromatography. Protein concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Description:
Secondary antibodies are raised in rabbit against human and animal immunoglobulin-G. Antibodies to human and animal whole serum are developed in rabbit. The evaluations of the antibodies are done using double Immunodiffusion, Immunoelectrophoresis, ELISA and Western blot and Quantitative precipitation assay.

Description:
BCIP/NBT (5-bromo-4-chloro-3-indolyl phosphate /nitro blue tetrazolium) is used in assay involving alkaline phosphatase enzyme. It produces insoluble bluish grey to black precipitate depending upon the enzyme concentration.

Description:
TMB/H202 [Tetramethyl benzidine / hydrogen peroxide] is used in assay involving Horseradish peroxidase enzyme. It produces insoluble bluish precipitate depending upon the enzyme concentration.

Description:
DAB/H202 [Diaminobenzidine tetrahydrochloride / hydrogen peroxide] is used in assay involving Horseradish peroxidase enzyme. It produces insoluble black precipitate.

Description:
TMB/H202 [Tetramethyl benzidine / hydrogen peroxide] is used in assay involving Horseradish peroxidase enzyme. It produces soluble blue colored product reacting with HRP. Reaction is stopped with H2S04 which results in yellow color. That is read at 450nm.

Description:
pNPP [pNitrophenlyl phosphate] is used in assay involving alkaline phosphatase enzyme. It produces soluble yellow colored product that can be read at 450nm.