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Microbial Identification Service |
Ordering Information: |
Product |
Cat. No |
Size |
Price |
Bacterial Identification service |
4018 |
Per sample |
Rs.7500/- |
Actinomycetes Identification service |
4029 |
Per sample |
Rs.7500/- |
Fungal Identification service |
4030 |
Per sample |
Rs.8500/- |
Yeast Identification service
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4031 |
Per sample |
Rs.8500/- |
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Introduction
Species identifications underpin all of biological research. Existing morphologically-based diagnostic approaches are often both cumbersome to use and are effective only for certain life stages. DNA-based systems promise to revolutionize the task of identification by providing reliable, inexpensive and rapid diagnosis of species identity.
Features:
Following protocols used for identification of microbes;
Bacterial Identification: 16S rDNA gene based – 1500bp
Actinomycetes Identification: Actinomycete species specific 16S rDNA – 750bp
Fungal Identification: ITS region based – 650bp
Yeast Identification: ITS region based – 500bp
Deliverables
• Detailed Report
• Protocol of DNA purification, PCR and Agarose gel electrophoresis
• Gel image - documentation
• DNA Sequencer - Chromatogram
• Nucleotide sequence alignment within 10 most similar Neighbours
• Phylogenetic tree displaying evolutionary relationship of 10 nearest neighbours
Delivery time
4 – 6 weeks
How to send sample:
Please contact us: Phone: 044-24491904/by mail to: helinibiomolecules@gmail.com
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DNA Barcoding Service |
Ordering Information: |
Product |
Cat. No |
Size |
Price |
Plants DNA barcoding service |
4032 |
Per sample |
Rs.7500/- |
Fish DNA barcoding service |
4033 |
Per sample |
Rs.7500/- |
Animal DNA barcoding service
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4034 |
Per sample |
Rs.8500/- |
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Introduction
Briefly, DNA barcoding is the use of a short gene sequence from a standardized region of the genome that can be used to help discover, characterize, and distinguish species, and to assign unidentified individuals to species.
Deliverables
• Detailed Report
• Protocol of DNA purification, PCR and Agarose gel electrophoresis
• Gel image - documentation
• DNA Sequencer - Chromatogram
• Nucleotide sequence alignment within 10 most similar Neighbours
• Phylogenetic tree displaying evolutionary relationship of 10 nearest neighbours
• Submission to NCBI (on request)
Delivery time
4 – 6 weeks
How to send sample:
Please contact us: Phone: 044-24491904/by mail to: helinibiomolecules@gmail.com
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Gene Expression studies Service (Real time PCR) |
Ordering Information: |
Product |
Cat. No |
Size |
Price |
qPCR analysis of control and sample includes
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4038 |
Per sample |
Rs.25000/- |
QPCR primer assay validation |
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Synthesis of primer |
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| RNA/mRNA purification & Quantification |
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| Documentation & Report |
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Introduction
Real-time PCR is mainly used to provide quantitative measurements of gene transcription. The technology may be used in determining how the genetic expression of a particular gene changes over time, such as in the response of tissue and cell cultures to an administration of a pharmacological agent, progression of cell differentiation, or in response to changes in environmental conditions .
Applications
• Validate microarray-derived gene expression data.
• Examine a focused panel of genes using multiple qPCR Primer Assays.
• Verify gene expression knock down by RNAi.
• Identify and confirm disease-associated biomarkers.
• Monitor phenotypic changes related to gene expression.
Delivery time
6 - 8weeks
How to send sample:
Please contact us: Phone: 044-24491904/by mail to: helinibiomolecules@gmail.com |
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HPLC Profiling Service |
Ordering Information: |
Product |
Cat. No |
Size |
Price |
HPLC profiling
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4039 |
Per sample |
Rs.4000/- |
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| Introduction
High-performance liquid chromatography (or High pressure liquid chromatography , HPLC ) is a form of column chromatography used frequently to separate, identify, and quantify compounds. HELINI offers HPLC profiling services for your crude sample along with standards. Various co mpounds in trace concentrations as low as parts per trillion [ppt] may easily be identified. HPLC can be applied to just about any sample, such as pharmaceuticals, food, cosmetics, environmental matrices, forensic samples, and industrial chemicals.
Deliverables:
• Detailed report
• Graph with protocol
Delivery time
2-3 weeks
How to send sample:
Please contact us: Phone: 044-24491904/by mail to: helinibiomolecules@gmail.com |
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Primer Designing Service |
Ordering Information: |
Product |
Cat. No |
Size |
Price |
Gene Specific Primer Designing service |
4035 |
Per sample |
Rs.1500/- |
Universal Primer Designing service |
4036 |
Per sample |
*Enquire |
Species Specific Primer Designing service
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4037 |
Per sample |
Rs.9500/- |
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Introduction
Successful PCR products are depends on the efficiency of primer. HELINI offers primer designing services.
Features:
• Accurate and analysed primer sequence.
• Appropriate Tm and GC content
• Validation report
Delivery time
4 – 6 weeks
More information: Please contact us: Phone: 044-24491904 / by mail to: helinibiomolecules@gmail.com
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Custom Oligonucleotide Services |
Ordering Information: |
Cat. No. |
Sample |
Pack |
Price |
4011 |
Desalted Oligo (upto 50base 2 OD) |
per base |
Rs.35 |
4012 |
Desalted Oligo (upto 50base 8 OD) |
per base |
Rs.125 |
4013 |
Purifications PAGE |
per Oligo |
Rs.1000 |
4014 |
HPLC Purification
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per Oligo |
Rs.4000 |
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Description
HELINI offers custom oligonucleotide service to scientists. The Oligonucleotide available as a desalted and PAGE purified. Oligonucleotide will be supplied with certificate of analysis.
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• Designed primer can be synthesized and
Presentation
Oligo supplied as a lyophilized form, after dilution store at -20ºC
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DNA Sequencing Services |
Ordering Information: |
Cat. No. |
Sample |
Pack |
Price |
4011 |
Up to 600 bases
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per sample
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Rs.
950 |
4012 |
Purification of PCR products and Sequencing up to 600 bases
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per sample |
Rs.1500 |
4013 |
Purification of plasmid and sequencing Up to 600 bases
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per sample |
Rs.1750 |
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Description
HELINI offers DNA sequencing service using latest and the highest quality high throughput technologies for its sequencing. Together with stringent quality assurance and quality control, we have been consistently receiving good feedbacks from our customers regarding the services.
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Sample accepted for sequencing
- Purified PCR sample
- Unpurified PCR sample
- Microbial culture having plasmids
- Purified Plasmids with gene of interest
- Unpurified Plasmids with gene of interest
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Polyclonal Antibody production services |
Ordering Information: |
Cat. No. |
Sample |
Pack |
Price |
4023 |
Custom made polyclonal antibodies in rabbit |
per animal |
Rs. 12000 |
4024 |
Purification of antibodies by ion exchange Chromatography
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up to 10mg |
Rs. 4500 |
4025 |
Purification of antibodies by affinity chromatography |
up to 10mg |
Rs. 5000 |
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Description
HELINI offers polyclonal antibodies service to scientists. Antibodies are raised in Rabbits. 25ml of immunized rabbit serum will be delivered to customers. Customer must send a purified protein samples in lyophilized or frozen form.
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Features
• Well standardized protocol
• Duration of 3 months
• Purifications services available
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Antibody labeling services |
Ordering Information: |
Cat. No. |
Sample |
Pack |
Price |
4026 |
Antibody labeling with HRP
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up to 10mg |
Rs. 4500 |
4027 |
Antibody labeling with ALP |
up to 5mg |
Rs. 20000 |
4028 |
Antibody labeling with Biotin |
up to 10mg |
Rs. 6500 |
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Description
HELINI offers antibody labeling services. Customer must send a purified protein samples. Method of labeling will be based on the enzyme and protein requirement.
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Requirement:
For HRP: 2 10mg of purified IgG or antibody
For ALP: 2 5mg of purified IgG or antibody
For Biotin: 2 10mg of purified IgG or antibody
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