Products - Modifying Enzymes : HELINI Biomolecules

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	Shrimp Alkaline phosphatase
	Calf Intestine Alkaline phosphatase
	DNA Polymerase I [Klenow fragment]

Shrimp Alkaline phosphatase

Ordering Information:
Cat. No.	Pack	Price (Rs.)
3106	100units	3200

Concentration
1U - 5U/µl

Supplied with
10X Reaction buffer

Storage buffer
20mM Tris-HCl (pH7.4), 0.1mM ZnCl2, 1mM MgCl2 and 50% glycerol

Storage
Store at -20ºC.

Inactivation
Inactivated by heating at 65ºC for 15min

Description
The Shrimp Alkaline phosphatase catalyzes the release of 5'- and 3' phosphate groups from DNA, RNA and nucleotides.

Applications
• Dephosphorylation of DNA and RNA
• Degradation of dNTPs in PCR mixture prior to sequencing of PCR products. • Dephosphorylation of proteins

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Calf Intestine Alkaline phosphatase

Ordering Information:
Cat. No.	Pack	Price (Rs.)
3105	100units	3200

Concentration
1U - 5U/µl

Supplied with
10X Reaction buffer

Storage buffer
20mM Tris-HCl (pH8.0), 50mM KCl, 0.1mM ZnCl2,
1mM MgCl2 and 50% glycerol

Storage
Store at -20ºC.

Inactivation
Inactivated by heating at 85ºC for 15min

Description
The Calf Intestine Alkaline phosphatase catalyzes the release of 5'- and 3' phosphate groups from DNA, RNA and nucleotides.

Applications
• Dephosphorylation of DNA and RNA
• Degradation of dNTPs in PCR mixture prior to sequencing of PCR products.
• Dephosphorylation of proteins

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DNA Polymerase I [Klenow fragment]

Ordering Information:
Cat. No.	Pack	Price (Rs.)
3109	50units	2200
3110	200units	4200

Concentration
2U- 10U/µl

Supplied with
10X Reaction buffer

Storage buffer
50mM potassium phosphate (pH7.4), 1mM DTT and 50% glycerol

Storage Store at
-20ºC.

Inactivation
Inactivated by heating at 75ºC for 10min or by addition of EDTA.

Description
The T4 Polynculeotide kinase is a polynucleotide 5'-hydroxyl kinase that catalyzes the transfer of the phosphate from ATP to the 5'-OH group of single and double stranded DNA and RNA, Oligonucleotides or nucleoside 3' monophosphates.

Applications
• Labeling 5'-termini of nucleic acids by either the forward or the exchange reaction.
• 5'-phosphorylation of oligonucleotide linkers and DNA or RNA prior to ligation.

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