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Taq DNA Polymerase |
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Ordering Information: |
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Cat. No. |
Pack |
Price (Rs.) |
5001
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100units
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1400 |
5002 |
250units
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2500 |
5003 |
1000units
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7000 |
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Concentration
5U/µl
Supplied with:
10X Taq reaction buffer containing 2mM MgCl2 10X Mg+ free Taq buffer
25mM MgCl2
Storage buffer
20mM Tris-HCl (pH8.0), 100mM KCl, 0.1mM EDTA, 1mM DTT, 0.5% Nonidet P40, 0.5% Tween 20 and 50% glycerol.
Storage:
Store at -20ºC.
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Description
The Taq DNA polymerase is a highly thermostable DNA polymerase from the thermophilic bacterium Thermus aquaticus . The enzyme catalyzes template dependent polymerization of nucleotides into duplex DNA in the 5'->3' direction. Taq DNA polymerase has no detectable 3'->5' exonuclease activity.
Applications
• PCR amplification of DNA fragments as long as 5kb.
• DNA labeling.
• DNA sequencing.
• Generation of PCR product for TA cloning.
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10X Taq DNA Polymerase Buffers |
Ordering Information: |
| Cat. No. |
Discription |
Qty. |
Price(Rs.) |
5006
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10X Taq buffer with KCl and 15mM MgCl2
Composition: 100mM Tris-HCl (pH 8.8 at 25ºC), 500mM KCl, 0.8% Nonidet P40 and 15mM MgCl2
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1ml
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750
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5007 |
10X Taq buffer with KCl
Composition: 100mM Tris-HCl (pH 8.8 at 25ºC), 500mM KCl, 0.8% Nonidet P40
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1ml |
750 |
5008 |
10X Taq buffer with (NH4)2SO4 and 20mM MgCl2
Composition: 750mM Tris-HCl (pH 8.8 at 25ºC), 200mM (NH4)2SO4, 0.1% Tween20, 20mM MgCl2
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1ml
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750 |
5009 |
10X Taq buffer with (NH4)2SO4
Composition:
750mM Tris-HCl (pH 8.8 at 25ºC), 200mM (NH4)2SO4, and 0.1% Tween20
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1ml |
750 |
5012 |
25mM MgCl2
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1ml |
750 |
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2X PCR Master Mix |
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Ordering Information: |
Cat. No. |
Pack |
Price (Rs.) |
5013 |
100reactions |
2600 |
5014 |
100reactions |
2600 |
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Recommended Protocol for PCR
• 2X Master Mix 25µl 10µl
• Add
Primer 1 1-2µl 1-2µl
Primer 2 1-2µl 1-2µl
DNA 1-5µl 1-5µl
Sterile deionized water to 50µl 20µl
• Mix gently and spin down for a few seconds.
• Place samples in a thermocycler and start PCR.
Storage: Store at -20ºC.
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Description
2X PCR Master Mix is a premixed 2X concentrated solution of Taq DNA polymerase, reaction buffer, MgCl2 and dNTPs. 2X PCR Master Mix contain all components for PCR, except DNA template and Primers. This mixture is optimized for consistent and efficient routine PCR amplifications.
Applications
• Routine PCR amplification with high reproducibility. |
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2X Redye® PCR Master Mix |
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Ordering Information: |
Cat. No. |
Pack |
Price (Rs.) |
5015 |
100reactions |
4500 |
5016 |
100reactions |
4500 |
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Recommended Protocol for PCR
• 2X Master Mix 25µl 10µl
• Add
Primer 1 1-2µl 1-2µl
Primer 2 1-2µl 1-2µl
DNA 1-5µl 1-5µl
Sterile deionized water to 50µl 20µl
• Mix gently and spin down for a few seconds.
• Place samples in a thermocycler and start PCR.
Storage: Store at -20ºC.
Single gene of 500bp amplified from Lambda DNA
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Description
2X Redye PCR Master Mix is a unique blend of premixed 2X concentrated solution of Taq DNA polymerase, reaction buffer, MgCl2, dNTPs and inert red dye. This dye enables amplified samples to load directly onto an agarose gel for electrophoresis followed PCR.
The red dye migrates slightly faster than bromophenol blue at about the same rate as a 125 bases pair fragment in a 1% agarose gel. Since no additional loading buffer are added to the reaction following PCR.
Advantage:
• Re-amplification is possible.
• Can be used for Nested PCR amplifications
• No additional loading dye is necessary, easy and can be directly loaded on to agarose gel.
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Confident ® Control Primer mix for Normal PCR [Human DNA] |
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Ordering Information: |
Cat. No. |
Pack |
Price (Rs.) |
5017 |
25Exp
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1500 |
5018 |
100Exp
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2500 |
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Features:
• Can be included as a control in normal PCR
• Optimum annealing temperature makes easy to run along with test samples.
• Ready to use
Quality Control:
The product is tested functionally using 2µl of DNA isolated from 50µl Blood sample using PCR-ReadyTemplate Genomic DNA preparation kit as the template for amplification of a 450bp segment of GAPDH gene (30 cycles).
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Description:
ConfidentControl® Primer mix is provided for use as a positive control in Normal PCR applications. It contains human GAPDH sense and anti-sense primers sufficient for 25/100 amplification reactions of 50µl each. The control primers produce a 450bp PCR product.
Kit components: 25Exp 100Exp
10µM Primer Mix 50µl 200µl
Instruction manual |
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Confident ® Control Primer mix for Normal PCR [Bacteria] |
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Ordering Information: |
Cat. No. |
Pack |
Price (Rs.) |
5019 |
25Exp
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1500 |
5020 |
100Exp |
2500 |
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Features:
• Can be included as a control in normal PCR
• Optimum annealing temperature makes easy to run along with test samples.
• Ready to use
Quality Control:
The product is tested functionally using 2µl of DNA isolated from E.coli using PCR-ReadyTemplate Genomic DNA preparation kit as the template for amplification of a 1500bp segment of 16S rRNA (30 cycles).
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Description:
ConfidentControl® Primer mix is provided for use as a positive control in Normal PCR applications. It contains Universal 16S rRNA sense and anti-sense primers sufficient for 25/100 amplification reactions of 50µl each. The control primers produce a 1500bp PCR product.
Kit components: 25Exp 100Exp
10µM Primer Mix 50µl 200µl
Instruction manual |
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Confident ® Control Primer mix for Normal PCR [Plant Chloroplast] |
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Ordering Information: |
Cat. No. |
Pack |
Price (Rs.) |
5019 |
25Exp |
1500 |
5020 |
100Exp |
2500 |
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Features:
• Can be included as a control in normal PCR
• Optimum annealing temperature makes easy to run along with test samples.
• Ready to use
Quality Control:
The product is tested functionally using 2µl of DNA isolated from leaf disk using PCR-ReadyTemplate Genomic DNA preparation kit as the template for amplification of a 400bp segment of Chloroplast gene (30 cycles).
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Description:
ConfidentControl® Primer mix is provided for use as a positive control in Normal PCR applications. It contains Plant chloroplast gene sense and anti-
Kit components: 25Exp 100Exp
10µM Primer Mix 50µl 200µl
Instruction manual
 Lane1: wheat; lane 2: maize; lane 3: oats; lane 4: barley; lane 5: 0.1・0-kb DNA markers.
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Confident ® Control Primer mix for Normal PCR [Fungi] |
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Ordering Information: |
Cat. No. |
Pack |
Price (Rs.) |
5023 |
25Exp |
1500 |
5024 |
100Exp |
2500 |
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Features:
• Can be included as a control in normal PCR
• Optimum annealing temperature makes easy to run along with test samples.
• Ready to use
Quality Control:
The product is tested functionally using 2µl of DNA isolated from leaf disk using PCR-ReadyTemplate Genomic DNA preparation kit as the template for amplification of a 400bp segment of Chloroplast gene (30 cycles).
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Description:
ConfidentControl® Primer mix is provided for use as a positive control in Normal PCR applications. It contains Fungi 18S rRNA sense and anti-sense primers sufficient for 25/100 amplification reactions of 50µl each. The control primers produce a 400bp PCR product.
Amplified from various Fungal DNA sample
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10mM dNTP Mix |
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Ordering Information: |
Cat. No. |
Pack |
Price (Rs.) |
3129
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100µl |
1100 |
3130
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250µl |
2100 |
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Applications
PCR, Long PCR, Real-time PCR, High fidelity PCR, RT-PCR, cDNA synthesis, Primer extension and DNA labeling.
Storage Store at -20ºC
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Description
dNTP mixes are aqueous solutions at pH 7.0 containing dATP, dCTP, dGTP and dTTP, each at a final concentration of 10mM and 2mM. dNTP mixes are ready to use solutions designed to save time and to provide higher reproducibility in PCR and other applications. |
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10mM dNTP set |
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Ordering Information: |
Cat. No. |
Pack |
Price (Rs.) |
5025
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4x100µl |
2500 |
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Applications
PCR, Long PCR, Real-time PCR, High fidelity PCR, RT-PCR, cDNA synthesis, Primer extension and DNA Labeling.
Storage Store at -20ºC
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Description:
dNTP set consists of 10mM aqueous solutions at pH7.0 of each of dATP, dCTP, dGTP and dTTP, each in a separate vial. |
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10mM dATP |
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Ordering Information: |
Cat. No. |
Pack |
Price (Rs.) |
5026 |
100µl |
1100 |
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Applications
For use in PCR, Long PCR, Real-time PCR, High fidelity PCR, RT-PCR, cDNA synthesis, Primer extension and DNA Labeling.
Storage Store at -20ºC
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Description: dATP (2'-deoxyadenosine 5'-triphosphate) is supplied as a 10mM aqueous solution at pH7.0
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10mM dTTP |
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Ordering Information: |
Cat. No. |
Pack |
Price (Rs.) |
5027 |
100µl |
1100 |
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Applications
For use in PCR, Long PCR, Real-time PCR, High fidelity PCR, RT-PCR, cDNA synthesis, Primer extension and DNA Labeling.
Storage Store at -20ºC
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Description: dTTP (2'-deoxyadenosine 5'-triphosphate) is supplied as a 10mM aqueous solution at pH7.0
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10mM dGTP |
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Ordering Information: |
Cat. No. |
Pack |
Price (Rs.) |
5028 |
100µl |
1100 |
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Applications
For use in PCR, Long PCR, Real-time PCR, High fidelity PCR, RT-PCR, cDNA synthesis, Primer extension and DNA Labeling.
Storage Store at -20ºC
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Description:
dGTP (2'-deoxyadenosine 5'-triphosphate) is supplied as a 10mM aqueous solution at pH7.0
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10mM dCTP |
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Ordering Information: |
Cat. No. |
Pack |
Price (Rs.) |
5029 |
100µl |
1100 |
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Applications
For use in PCR, Long PCR, Real-time PCR, High fidelity PCR, RT-PCR, cDNA synthesis, Primer extension and DNA Labeling.
Storage Store at -20ºC
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Description:
dCTP (2'-deoxyadenosine 5'-triphosphate) is supplied as a 10mM aqueous solution at pH7.0 |
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Oligo(dT) 15 Primer |
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Ordering Information: |
Cat. No. |
Pack |
Price (Rs.) |
5036 |
50-100reactions |
1000 |
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Concentration: 100uM/µl
Application: First strand cDNA synthesis
Storage: Store at -20ºC.
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Description
This primer is a mixture of single stranded random hexanucleotides with 5' and 3' hydroxyl ends. The primer is supplied as a ready to use.
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Oligo(dT) 16 Primer |
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Ordering Information: |
Cat. No. |
Pack |
Price (Rs.) |
5037 |
50-100reactions |
1000 |
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Concentration: 100uM/µl
Application: First strand cDNA synthesis
Storage: Store at -20ºC.
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Description
This primer is a single stranded 16mer oligonucleotide with 5' and 3' hydroxyl ends. The primer is supplied as a ready to use.
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Oligo(dT) 18 Primer |
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Ordering Information: |
Cat. No. |
Pack |
Price (Rs.) |
5038 |
50-100reactions |
1000 |
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Concentration:100uM/µl
Application:First strand cDNA synthesis
Storage: Store at -20ºC
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Description
This primer is a single stranded 18mer oligonucleotide with 5' and 3' hydroxyl ends. The primer is supplied as a ready to use.
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Oligo(dT) 20 Primer |
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Ordering Information: |
Cat. No. |
Pack |
Price (Rs.) |
5039 |
50-100reactions |
1000 |
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Concentration:100uM/µl
Application:First strand cDNA synthesis
Storage: Store at -20ºC |
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Description
This primer is a single stranded 20mer oligonucleotide with 5' and 3' hydroxyl ends. The primer is supplied as a ready to use.
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Random Primer (10mer) |
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Ordering Information: |
Cat. No. |
Pack |
Price (Rs.) |
5034 |
50-100reactions |
1000 |
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Concentration: 100uM/µl
Application: Random amplified Polymorphic DNA [RAPD]
Storage: Store at -20ºC.
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Description
This primer is a single stranded 1mer oligonucleotide. The primer is supplied as a ready to use.
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Primers |
Recommended
Final Concentration
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Random primer
Oligo (dT) primer
Random Hexamer
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5-10pmoles/µl
25ng/µl
10ng/µl
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Ribonuclease inhibitor (from human placenta) |
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Ordering Information: |
Cat. No. |
Pack |
Price (Rs.) |
3165 |
500units |
2000 |
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Concentration: 20-50U/µl
Storage: Store at -20ºC. |
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Description
The Ribonuclease inhibitor inhibits the activity of RNases A, B and C by binding them in a non competitive mode at a 1:1 ratio.
Application
Inhibition of RNA degradation |
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Pfu DNA Polymerase |
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Ordering Information: |
Cat. No. |
Pack |
Price (Rs.) |
5004 |
100units |
6500 |
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Concentration 2-5U/µl
Supplied with:
10X Pfu reaction buffer containing 20mM MgCl2
10X Mg+ free Pfu buffer
25mM MgCl2
Storage buffer
20mM Tris-HCl (pH8.0), 100mM KCl, 0.1mM EDTA, 1mM DTT, 0.5% Nonidet P40, 0.5% Tween 20 and 50% glycerol.
Storage: Store at -20ºC. |
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Description
Pfu DNA polymerase is a DNA polymerase of Pyrococcus furiosus , a hyperthermophiic archaebacterium. The enzyme catalyzes the incorporation of nucleotides into duplex DNA in the 5'->3' direction in the presence of magnesium ions at 70ºC-80ºC. Unlike Taq DNA polymerase, Pfu DNA polymerase exhibits 3'->5' exonuclease activity. (proof reading) that enables the polymerase to correct nucleotide incorporation errors.
Applications
• All PCR amplification which demand high fidelity.
• Site directed mutagenesis.
• High fidelity PCR for cloning into blunt-ended vectors. |
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M-MuLV Reverse Transcriptase |
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Ordering Information: |
Cat. No. |
Pack |
Price (Rs.) |
5030 |
500units |
3900 |
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Concentration 10-25U/µl
Supplied with: 5X Reaction buffer
Storage buffer
50mM Tris-HCl (pH8.3), 0.1M NaCl, 1mM EDTA, 5mM DTT, 0.1% Tween 20 and 50% glycerol.
Storage Store at -20ºC.
Inactivation
Inactivation by heating at 70ºC for 10min |
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Description
The M-MuLV Reverse Transcriptase is an RNA and DNA dependent DNA polymerase. It can use either RNA or DNA to primer DNA synthesis. The enzyme possesses a ribonuclease H activity specific to RNA in RNA-DNA hybrids.
Applications
• First strand cDNA synthesis
• Use with PCR and Real time PCR
• DNA labeling
• Second strand cDNA synthesis |
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Hot Start Taq DNA Polymerase |
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Ordering Information: |
Cat. No. |
Pack |
Price (Rs.) |
5005 |
100units |
3900 |
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Concentration 2-5U/µl
Supplied with:
10X Mg+ free Hot Start Taq buffer
25mM MgCl2
Storage buffer
20mM Tris-HCl (pH8.0), 100mM KCl, 0.1mM EDTA, 1mM DTT, 0.5% Nonidet P40, 0.5% Tween 20 and 50% glycerol.
Storage:
Store at -20ºC. |
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Description
Hot Start PCR is a technique that has been used to improve specificity, sensitivity and yield of DNA amplification during PCR.
Hot Start Taq DNA polymerase is a Taq DNA polymerase which has been chemically modified by the addition of proprietary heat-liable blocking groups to amino acid residues. Due to this modification, Taq DNA polymerase is inactive at room temperature, avoiding extension of non-specifically annealed primers or primer dimers. Hot start Taq DNA Polymerase is instantaneously activated during the initial denaturation step of PCR. It catalyzes 5'__3' synthesis of DNA, has no detectable 3'__5' exonuclease activity, but possesses low 5'__3' exonuclease activity. |
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Primers for Sequencing |
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Description
This primer is a single stranded 18mer oligonucleotide with 5' and 3' hydroxyl ends. The M13/pUC sequencing primer anneal to the region in the 5' terminus of the lacZ gene is supplied as a ready to use.
Concentration
10pmoles/µl
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Application
Sequencing of DNA fragments inserted into the MCS (Multi-cloning site) within the lacZ gene of various cloning vectors, such as pUC19, pUC18, pBluescript-II, M13mp18.
Colony screening by PCR
Storage: Store at -20ºC.
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Ordering Information: |
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Sequencing Primer |
Cat. No. |
Qty |
Price (Rs.) |
M13/pUC sequencing primer (-20) 17mer |
5040 |
100reactions |
1000 |
M13/pUC reverse sequencing primer (-26) 17mer |
5041 |
100reactions |
1000 |
M13/pUC sequencing primer (-40) 17mer |
5042 |
100reactions |
1000 |
M13/pUC sequencing primer (-46) 22mer |
5043 |
100reactions |
1000 |
M13/pUC reverse sequencing primer (-46) 24mer |
5044 |
100reactions |
1000 |
pBR EcoRI site primer -16mer |
5045 |
100reactions |
1000 |
pBR HindIII site primer -20mer |
5046 |
100reactions |
1000 |
T7 Universal primer -20mer |
5047 |
100reactions |
1000 |
T7 Terminator primer -19mer |
5048 |
100reactions |
1000 |
T3 ・20mer |
5049 |
100reactions |
1000 |
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