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Polyacrylamide Gel mix for SDS PAGE |
Separating gel mix |
Cat. No. |
Product Discription |
Pack |
Price (Rs.) |
3185
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8%
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100ml |
1300 |
| 3143 |
10% |
100ml |
1300 |
| 3142 |
12% |
100ml |
1400 |
| 3141 |
15% |
100ml |
1500 |
Stacking gel mix |
| 3144 |
5% |
100ml |
1300 |
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Storage 2-8ºC
Recommended usage:
Add freshly prepared APS to Gel mix and immediately use. |
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Description
Polyacrylamide Ready gel mix solutions for use in Denaturing SDS Polyacrylamide electrophoresis are available in most common ratios. They form polyacrlyamide gel on addition of APS or Riboflavin
Features
• Eliminates the need to weight toxic acrylamide and bis-acrylamide
• Solution prepared from electrophoresis grade reagents in ultra pure water and filter sterilized
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Polyacrylamide Gel mix for Native PAGE |
Cat. No. |
Product Discription |
Pack |
Price (Rs.) |
Separating gel mix |
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| 3186 |
10% |
100ml |
1200 |
Stacking gel mix |
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| 3187 |
5% |
100ml |
1200 |
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Description
Polyacrylamide Ready gel mix solutions for use in Native Polyacrylamide electrophoresis are available in most common ratios. They form polyacrlyamide gel on addition of APS or Riboflavin
Features
• Eliminates the need to weight toxic acrylamide and bis-acrylamide.
• Solutions prepared from electrophoresis grade reagents in ultra pure water and filter sterilized. |
Storage 2-8ºC
Recommended usage:
Add freshly prepared 10%APS to gel mix and use immediately. |
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10X Tris glycine SDS buffer |
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Ordering Information: |
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Cat. No. |
Pack |
Price (Rs.) |
3147 |
1lit |
2000 |
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Composition
Dilution of 10X buffer produces a 1X running buffer containing 25mM Tris, 192mM glycine, 0.1% SDS, pH approx 8.6.
Recommended usage
Dilute with deionized water in 1:9 ratio (1X concentration)
For each electrophoresis fresh 1X buffer should be prepared
Recommended running in 100 Volts for mini vertical gel electrophoresis unit.
Storage
Store at 2 - 8ºC
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Description
Tris-glycine-SDS running buffer is the most commonly used buffer for sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) of proteins. It is usually used for both the anode buffer and the cathode buffer.
Application
Used as a running tank buffer in denaturing polyacrylamide gel electrophoresis of proteins. (SDS-PAGE)
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10X Tris glycine buffer |
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Ordering Information: |
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Cat. No. |
Pack |
Price (Rs.) |
3155 |
1lit |
2000 |
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Composition
Dilution of 10X buffer produces a 1X running buffer containing 25mM Tris, 192mM glycine, pH approx 8.3.
Recommended usage
Dilute with deionized water in 1:9 ratio (1X concentration)
For each electrophoresis fresh 1X buffer should be prepared
Recommended running in 100 Volts for mini vertical gel electrophoresis unit.
Storage Store at
2 - 8ºC
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Description
Tris-glycine running buffer is the most commonly used buffer for Native polyacrylamide gel electrophoresis (Native-PAGE) of proteins. It is usually used for both the anode buffer and the cathode buffer.
Application
Used as a running tank buffer in Native polyacrylamide gel electrophoresis of proteins. (Native-PAGE)
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10X Tris tricine SDS buffer |
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Ordering Information: |
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Cat. No. |
Pack |
Price (Rs.) |
3157 |
500ml. |
2200 |
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Composition
1X running buffer containing 100mM Tris, 100mM tricine, 0.1% SDS, pH approx 8.3
Recommended usage
Dilute with deionized water in 1:9 ratio (1X concentration)
For each electrophoresis fresh 1X buffer should be prepared
Recommended running in 100 Volts for mini vertical gel electrophoresis unit.
Storage
Store at 2 - 8ºC
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Description
Tris-tricine-SDS running buffer is the most commonly used in denaturing polyacrylamide gel electrophoresis of low molecular weight proteins and peptides.
Application
Running buffer in Denaturing polyacrylamide gel electrophoresis of low molecular weight proteins (SDS-PAGE) |
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1.5M Tris SDS buffer (pH8.8) |
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Ordering Information: |
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Cat. No. |
Pack |
Price (Rs.) |
3158 |
100ml |
1450 |
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Application Prep
aration of Polyacrylamide separating gel mix
Storage
Store at room temperature
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Description
1.5M Tris SDS buffer is the used in the preparation of polyacrylamide gel electrophoresis resolving or separating gel mix. Buffer prepared with molecular biology grade chemicals.
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1.0M Tris SDS buffer (pH6.8) |
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Ordering Information: |
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Cat. No. |
Pack |
Price (Rs.) |
3159 |
100ml |
1200 |
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Application
Used in the preparation of Polyacrylamide stacking gel mix
Storage
Store at room temperature
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Description
1.0M Tris SDS buffer is the used in the preparation of polyacrylamide gel electrophoresis stacking gel mix.
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Commassie Staining solution |
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Ordering Information: |
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Cat. No. |
Pack |
Price (Rs.) |
3145 |
500ml |
1800 |
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Composition
Commassie staining solution contains 0.3% Commassie brilliant blue R-250, 50% ethanol and 10% acetic acid.
Recommended usage
Cover gel with Commassie staining solution and incubate for 2 hours with gentle shaking.
Storage
Store at 2 - 8ºC
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Description
Commassie staining solution is commonly used stain to stain Native and Denatured polyacrlyamide gel. Destaining is required to see the bands.
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Destaining solution |
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Ordering Information: |
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Cat. No. |
Pack |
Price (Rs.) |
3146 |
1lit |
1600 |
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Composition
Destaining solution contains 5% ethanol and 7% acetic acid.
Recommendation;
Cover gel with destaining solution for overnight. Change the destaining solution if required.
Storage
Store at room temperature
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Description
Destaining solution is used destain the gel which is stained with Commassie staining solution in both Native and Denatured polyacrlyamide gel. |
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2X Western Blotting Transfer Buffer |
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Ordering Information: |
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Cat. No. |
Pack |
Price (Rs.) |
3150 |
100ml |
950 |
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Composition
1X Western blotting buffer containing 15mM Tris, 120mM glycine, 0.3% SDS and 5% methanol, pH approx 7.8
Recommended usage
Dilute with deionized water in 1:1 ratio
For each electrophoresis fresh 1X buffer should be prepared
Storage
Store at 2 - 8ºC
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Description
2X Western blotting transfer buffer is used in transfer proteins from gel to membrane. Buffer can be used for both semi dry and tank blot method of transfer.
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BlueFast® protein staining solution |
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Ordering Information: |
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Cat. No. |
Pack |
Price (Rs.) |
3149 |
100ml |
1700 |
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Features
• Highly sensitive 5ng of protein can be easily detected.
• Quantitative linear detection signal over 5-500ng range.
• Ready-to-use.
• Economical, can be reused up to three times.
• Compatible with MALDI or other methods of mass spectrometry and protein micro-sequencing. • Rapid, no destaining required
• Safe does not contain any toxic components.
• End point stain, over-staining does not occur.
• Gel-to-gel reproducibility.
• Convenient packaging.
• Stable at room temperature for 12months.
Storage
Stable at 4 to 26ºC
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Staining Protocol
For mini gels (8x10cm 0.5mm thickness )
Place the gel into a tray; add 100ml of deionized water
Incubate for 10 min
Discard the wash and repeat 3 times
Add 20 to 30ml of BlueFast® Protein staining solution and stain with gentle agitation for 1 hour to overnight
(For 1mm and 1.5mm gel, incubate the gel overnight for higher sensitivity)
Decant the staining solution in a separate container for reuse
Add 100ml of deionized water and incubated for 15 minutes
Discard wash; and repeat thrice
Add 100ml of deionized water for gel storage |
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Description
The BlueFast® Protein Staining Solution is a ready-to-use solution for highly sensitive detection of proteins on polyacrylamide gels. The BlueFast is based on the Commassie Brillant Blue G-250 dye.
This solution preferentially stains proteins with little background on the gel matrix. Proteins are stained to an end point, therefore, over staining does not occur even after overnight staining. The BlueFast® generates a superior detection signal for reliable protein quantification over a 5-500ng range.
Due to the unique formulation of a BlueFast® as little as 5ng of protein can be detected. This is approximately 10-fold more sensitive than with Coomassie Brilliant Blue R-250 based stains and is close to that of silver staining.
The BlueFast® Protein Staining Solution does not contain toxic methanol and acetic acid. It is safe and environmentally friendly. The BlueFast® Protein Staining Solution does not require expensive and hazardous de-staining reagents.
This solution can be reused up to three times without any loss in the staining sensitivity. Protein staining with BlueFast® is an economical, fast and highly sensitive procedure.
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2X Sample loading buffer |
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Ordering Information: |
Cat. No. |
Pack |
Price (Rs.) |
3148 |
20ml |
1400 |
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Composition
Solution contains 4% SDS, 20% glycerol, 10% 2-mercaptoethoanl, 0.004% Bromophenol blue and 0.125M Tris HCl (pH 6.8).
Recommended usage:
Add 1 volume gel loading solution to 1 volume sample and mix well.
Heat in boiling water for 2-5min and cool to room temperature.
Storage
Store at room temperature
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Description
The Sample loading buffer is used for preparation of protein samples prior to denaturing SDS Polyacrylamide gel electrophoresis.
Applications
Preparation of proteins for SDS-Polyacrylamide gel electrophoresis (SDS-PAGE)
Preparation of total protein from various biological sources for SDS PAGE
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Ponceu U Staining solution |
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Ordering Information: |
Cat. No. |
Pack |
Price (Rs.) |
3151 |
100ml |
800 |
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Recommended usage:
Procedure for PVDF And Nitrocellulose Membranes:
Total Protein Detection:
• After transferring proteins from SDS-PAGE onto membrane, immerse the membrane in sufficient
• Ponceau S Staining Solution and stain for 5 minutes.
• Rinse membrane with distilled water until the background is clear.
• Dry the membrane under a heating fan.
Reversible Protein Detection:
• After proteins are transferred onto membrane, immerse the membrane in Ponceau S Staining Solution and stain for 5 minutes.
• After proteins have been visualized, rinse the membrane with distilled water and rapidly immerse in an aqueous solution of 0.1 M NaOH. Protein bands will start to disappear after 10-30 seconds.
• Rinse membrane with running distilled water for 2-3 minutes.
• Continue with other procedure being followed (e.g. immunological detection).
Storage
Store at 2 - 8ºC
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Description
Ponceau S Staining Solution is used for the detection of proteins on cellulose acetate, PVDF, and nitrocellulose membranes. For PVDF and nitrocellulose membranes, microgram quantities of transferred protein can be detected with a clear background and red protein bands. This staining technique is reversible to allow further immunological detection. The limit of detection for this stain is 250 nano grams of protein after separation by electrophoresis in polyacrylamide gels and transferred to nitrocellulose membranes
Application
• Rapid staining and detection of proteins on cellulose acetate, PVDF, and nitrocellulose membranes
• Reversible staining of proteins transferred on to nitrocellulose membrane, Cellulose acetate and PVDF.
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Prestained Protein Molecular Weight Marker |
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Ordering Information: |
Cat. No. |
Pack |
Price (Rs.) |
3139 |
0.2ml |
2200 |
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Composition
Approximately 0.2 mg/ml of each protein in 62.5mM Tris-HCl (pH 7.5 at 25°C), 1mM EDTA, 2% SDS, 10mM DTT, 1.5mM NaN 3 and 33% glycerol.
Storage
Store at -20°C. |
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Description
The Prestained Protein Molecular Weight Marker is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency and approximate sizing of proteins. The marker is a mixture of 6 proteins with the apparent molecular weights from 20 kDa to 120 kDa.
Application
• Monitoring protein separation during SDS PAGE
• Verification of Western transfer efficiency in membrane.
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Protein Molecular Weight Marker (Ready to use) |
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Ordering Information: |
Cat. No. |
Pack |
Price (Rs.) |
3139 |
0.2ml |
2200 |
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Instruction for Use
• Thaw the marker at room temperature or heat for few minutes at 37ºC. Vortex gently to ensure the solution is homogeneous.
• It is recommended to divide the marker into several aliquots to avoid contamination of the stock solution.
• Heat this tube at 95ºC for 5 minutes for complete denaturation of proteins. Cooled and mixed solution is ready to loading on an SDS PAGE gel.
• Load the marker on an SDS-PAGE gel and run
• Coomassie or other protein staining methods can be used to visualize the marker.
• Store the denatured marker at -20ºC
• For further loading thaw the marker at room temperature for a few minutes, then vortex. Do not heat the marker at higher temperature.
Storage
Store at -20ºC |
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Description
The Protein Ladder is designed for accurate sizing of proteins by SDS-Polyacrlyamide gel electrophoresis. It is a mixture of proteins which appears as sharp bands after SDS-Polyacrlyamide gel electrophoresis when stained.
Features
• Sharp bands
• Supplied in a loading buffer for direct loading on gels
Concentration
0.1 0.2mg/ml of proteins
Recommended loading volume
Loading volume |
Gel thickness |
5µl |
0.5mm 0.75mm thick mini gel |
| 10µl |
1.5mm thick mini gel |
| 20µl |
1.5mm thick large gel |
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