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RNase A (DNase Free) |
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Ordering Information: |
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Cat. No. |
Pack |
Price (Rs.) |
3111
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0.5ml
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1200 |
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Concentration
10mg/ml
Applications
Plasmid and genomic DNA preparation
Recommended working Concentration
1-100µg/ml
Storage
Store at -20ºC
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Description
Ribonuclease A (RNase A) is an endoribonuclease that specifically degrades single stranded RNA at C and U residues.
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Proteinase K |
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Ordering Information: |
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Cat. No. |
Pack |
Price (Rs.) |
3112 |
0.5ml |
1900 |
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Concentration
20mg/ml
Applications
Removal of DNases and RNases when isolating DNA and RNA
Recommended working concentration
0.02-1mg/ml at 65ºC
Storage
Store at -20ºC
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Description
Proteinase K is an endolytic protease that cleaves peptide bonds at the carboxylic slides of aliphatic, aromatic or hydrophobic amino acids.
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DNase I |
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Ordering Information: |
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Cat. No. |
Pack |
Price (Rs.) |
3113 |
0.5ml |
2250 |
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Concentration
5mg/ml
Applications
Prep
aration of DNA free RNA
Recommended working concentration
2U of DNase per 1µg of DNA at 37ºC
Storage
Store at -20ºC
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Description
Deoxyribonuclease I (DNase I) is an endonuclease that digest single and double stranded DNA.
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Lysozyme |
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Ordering Information: |
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Cat. No. |
Pack |
Price (Rs.) |
3114 |
500mg |
2100 |
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Applications
Lysing gram positive and gram negative bacteria for isolation of nucleic acid
Recommended working concentration
1mg/ml
Storage
Store at -20ºC
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Description
Lysozyme is a family of enzymes which damage bacterial cell walls by catalyzing hydrolysis of 1,4-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins.
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Ribonuclease Inhibitor (from Human placenta) |
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Ordering Information: |
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Cat. No. |
Pack |
Price (Rs.) |
3165 |
500u |
2000 |
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Concentration
10U-50U/µl
Applications
Inhibition of RNA degradation
Recommended working Concentration
1unit per µl of a reaction mixture
Storage
Store at -20ºC
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Description
The Ribonuclease Inhibitor inhibits the activity of RNase A, B and C by binding them in a non competitive mode at 1:1 ratio.
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Water - Nuclease free |
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Ordering Information: |
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Cat. No. |
Pack |
Price (Rs.) |
3162 |
1ml |
250 |
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Features
Free of endodeoxyribonucleases, exodeoxyribonucleases, ribonuclease and phosphatases.
Storage
Store at -20ºC
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Description
The water nuclease free is deionized and 0.22µm membrane filtered. It is suitable for use in all molecular biology applications.
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Water - DEPC treated |
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Ordering Information: |
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Cat. No. |
Pack |
Price (Rs.) |
3163 |
1ml |
350 |
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Features
Free of endodeoxyribonucleases, exodeoxyribonucleases, ribonuclease and phosphatases.
Applications
Used any application where RNA is involved
Storage
Store at -20ºC
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Description
The DEPC treated water is deionized, diethylpyrocarbonate (DEPC) treated and 0.22µm membrane filtered.
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10% SDS |
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Ordering Information: |
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Cat. No. |
Pack |
Price (Rs.) |
3166 |
100ml |
1200 |
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Features
Prepared using molecular biology grade chemical
Storage
Store at room temperature |
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Description
Sodium dodecyl sulphate ( SDS ) (C 12 H 25 SO 4 Na) is an anionic surfactant that is used in nucleic acid purifications.
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Potassium acetate 3M |
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Ordering Information: |
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Cat. No. |
Pack |
Price (Rs.) |
3167 |
100ml |
1200 |
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Applications
In nucleic acid preparation, potassium acetate is used to precipitate dodecyl sulfate (SDS) and SDS bound proteins, allowing the removal of proteins from DNA. It is also used as a salt for the ethanol precipitation of DNA.
Storage
Store at -20ºC
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Description
Potassium acetate 3M (pH 5.2) is used in DNA precipitation process. It is prepared using molecular biology reagents.
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Sodium acetate 3M |
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Ordering Information: |
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Cat. No. |
Pack |
Price (Rs.) |
3168 |
100ml |
1200 |
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Applications
In nucleic acid preparation, potassium acetate is used to precipitate dodecyl sulfate (SDS) and SDS bound proteins, allowing the removal of proteins from DNA. It is also used as a salt for the ethanol precipitation of DNA.
Storage
Store at -20ºC |
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Description
Sodium acetate 3M (pH 5.2) is used in DNA precipitation process. It is prepared using molecular biology reagents.
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Tris Saturated Phenol (pH~8.0) |
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Ordering Information: |
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Cat. No. |
Pack |
Price (Rs.) |
3169 |
100ml |
1250 |
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Applications
For DNA isolation, a pH of 7.58.0 is required, and both DNA and RNA will partition into the upper aqueous phase.
Used in the purification of nucleic acids.
Storage
Store at -20ºC
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Description
Phenol is a crystalline solid at room temperature. It is normally either saturated with an aqueous buffer or dissolved in chloroform to make it liquid. Phenol extraction is a commonly used method for deproteinization of nucleic acids. |
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Water Saturated Phenol (pH~4.5) |
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Ordering Information: |
Cat. No. |
Pack |
Price (Rs.) |
7072 |
100ml |
1250 |
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Applications
For RNA isolation, a pH of 4.55.0 is required, and RNA will partition into the upper aqueous phase.
Used in the purification of RNA.
Storage
Store at -20ºC
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Description
Phenol is a crystalline solid at room temperature. It is normally either saturated with an aqueous buffer or dissolved in chloroform to make it liquid. Phenol extraction is a commonly used method for deproteinization of nucleic acids.
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Phenol:Chloroform:Isoamyl alcohol |
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Ordering Information: |
Cat. No. |
Pack |
Price (Rs.) |
3164 |
50ml |
2500 |
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Description
Phenol:Chloloform:Isoamyl Alcohol (25:24:1) is saturated with Tris for the optimum pH~8.0 for DNA extractions.
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Phenol:Chloroform |
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Ordering Information: |
Cat. No. |
Pack |
Price (Rs.) |
3170 |
50ml |
2500 |
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Description
Phenol:Chloloform:Isoamyl Alcohol (5:1) is used for RNA extractions.
Storage
Store at 2 - 8ºC
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10X TE |
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Ordering Information: |
Cat. No. |
Pack |
Price (Rs.) |
3154 |
100ml |
1250 |
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Features
Prepared using molecular biology grade chemicals.
Storage
Room temperature
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Description
1X TE (Tris EDTA buffer pH8.0) is used as a rehydrating solution in DNA extraction that prevents DNA degradation.
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0.5M EDTA |
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Ordering Information: |
Cat. No. |
Pack |
Price (Rs.) |
| 3171 |
50ml |
950 |
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Features
Prepared using molecular biology grade EDTA disodium salt.
Storage
Room temperature
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Description
0.5M EDTA is a chelator of divalent cations that added in the buffers used for the preparation of nucleic acids to prevent from endonuclease activities.
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5M NaCl |
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Ordering Information: |
Cat. No. |
Pack |
Price (Rs.) |
3172 |
100ml |
1200 |
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1M Tris pH 8.0 |
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Ordering Information: |
Cat. No. |
Pack |
Price (Rs.) |
3173 |
100ml |
1400 |
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Description
Prepared using Molecular biology grade chemical and pH adjusted to 8.0. Suitable for all molecular biology experiments.
Storage: Room temperature |
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1M Tris pH 7.5 |
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Ordering Information: |
Cat. No. |
Pack |
Price (Rs.) |
3174 |
100ml |
1400 |
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Description
Prepared using Molecular biology grade Tris and pH adjusted to 7.5. Suitable for all molecular biology experiments.
Storage: Room temperature |
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OhmSol Reagent RNA isolation reagent |
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Ordering Information: |
Cat. No. |
Pack |
Price (Rs.) |
3175 |
25ml |
2500 |
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Features
Single step isolation of total RNA
Rapid and economical
Storage
2-8ºC |
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Description
OhmSol is a single step total RNA isolation reagent. It is ideal for quick, economical, and efficient isolation of total RNA form samples of human, animal, plant, yeast, bacterial and viral origin. |
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PRESERVE RNA storage |
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Ordering Information: |
Cat. No. |
Pack |
Price (Rs.) |
3176 |
50ml |
2900 |
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Features
Suitable for longer storage
Stable & recoverable RNA
Storage
2-8ºC
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Description
PRESERVE-RNA storage solution is an aqueous, non-toxic tissue storage reagent that rapidly permeates tissues to stabilize and protect cellular RNA. It eliminates the need to immediately process tissue samples or to freeze samples in liquid nitrogen for later processing.
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